ANNNOVAR variants

Pileup Indel

1. Sample data

chr10   57162   D1      G       *       homo    26      3       6
chr10   62899   I4      AAAA    *       hete    31      17      33
chr10   85429   I1      A       *       homo    38      29      32
chr10   87126   I24     TGCATTTACGTGATCTTGGCTCAC        *       hete    51      10      38
chr10   87668   D3      CTC     *       hete    52      27      34
chr10   89301   D1      A       *       hete    37      7       45
chr10   89448   I3      AGG     *       hete    27      4       31
chr10   93681   I1      G       *       hete    21      12      111
chr10   94117   I3      CAA     *       hete    29      29      81
chr10   94848   D3      TTA     *       hete    54      7       48
chr10   95775   I1      T       *       hete    44      6       44
chr10   97572   D1      T       *       hete    44      8       55
chr10   98719   I1      T       *       hete    48      13      39
chr10   99022   I1      T       *       homo    52      19      36
chr10   100224  D6      CCCTAA  *       hete    41      12      31
chr10   100433  D6      ACCCTC  *       hete    50      2       20
chr10   100799  I1      G       *       hete    50      4       22
chr10   101382  D1      G       *       hete    54      12      39
chr10   101729  D3      GTA     *       hete    51      19      58
chr10   103093  D1      T       *       homo    57      23      33
chr10   103731  D2      GA      *       hete    46      6       28
chr10   106207  D9      TTGTTTTTG       *       hete    46      6       24
chr10   106216  D4      TTTT    *       homo    49      11      19
chr10   107344  I1      C       *       hete    54      6       32
chr10   108119  I1      G       *       hete    31      7       19
chr10   108176  I1      A       *       hete    46      3       22
chr10   110565  D2      AA      *       hete    47      4       11
chr10   110582  D2      AG      *       hete    51      2       13
chr10   110806  D7      TTTTTTT *       hete    55      5       14
chr10   110829  I3      GGG     *       hete    45      2       13
chr10   111125  I1      T       *       homo    52      11      14
chr10   112581  D2      CC      *       homo    30      8       8
chr10   113972  D1      G       *       hete    56      7       40
chr10   114040  I1      A       *       hete    57      4       33
chr10   114710  D1      C       *       hete    56      4       28
chr10   117629  I8      CCAGATCC        *       hete    42      4       27
chr10   123201  D1      C       *       homo    58      20      22

2. Format description

vtools show format pileup_indel

INFO: Opening project test.proj
Format:      pileup_indels
Description: Input format for samtools pileup indel caller. This format imports
    chr, pos, ref, alt and genotype.

variant fields:
  chr:    Chromosome name
  pos:    Start position of the indel event.
  ref:    reference allele, '-' for insertion
  alt:    alternative allele, '-' for deletion

Genotype field:
   genotype:    type of indel (homozygote or heterozygote)

3. Example

vtools import --format pileup_indel pileup.indel --build hg18 

INFO: Opening project test.proj
INFO: Additional genotype fields: genotype
INFO: Importing genotype from pileup.indel (1/1)
DEBUG: Creating table sample
pileup.indel: 30
INFO: 30 new variants from 30 records are imported, with 0 SNVs, 14 insertions, 16 deletions, and 0 complex variants.


vtools show table variant -l -1

INFO: Opening project test.proj
variant_id, bin, chr, pos, ref, alt
1, 585, 10, 57162, G, -
2, 585, 10, 62899, -, AAAA
3, 585, 10, 85429, -, A
4, 585, 10, 87126, -, TGCATTTACGTGATCTTGGCTCAC
5, 585, 10, 87668, CTC, -
6, 585, 10, 89301, A, -
7, 585, 10, 89448, -, AGG
8, 585, 10, 93681, -, G
9, 585, 10, 94117, -, CAA
10, 585, 10, 94848, TTA, -
11, 585, 10, 95775, -, T
12, 585, 10, 97572, T, -
13, 585, 10, 98719, -, T
14, 585, 10, 99022, -, T
15, 585, 10, 100224, CCCTAA, -
16, 585, 10, 100433, ACCCTC, -
17, 585, 10, 100799, -, G
18, 585, 10, 101382, G, -
19, 585, 10, 101729, GTA, -
20, 585, 10, 103093, T, -
21, 585, 10, 103731, GA, -
22, 585, 10, 106207, TTGTTTTTG, -
23, 585, 10, 106216, TTTT, -
24, 585, 10, 107344, -, C
25, 585, 10, 108119, -, G
26, 585, 10, 108176, -, A
27, 585, 10, 110565, AA, -
28, 585, 10, 110582, AG, -
29, 585, 10, 110806, TTTTTTT, -
30, 585, 10, 110829, -, GGG